Descriptions, field collections
Library prep of 74 x Sterivex DNA extracts (51 true samples, 20 FLD NEGs, 3 EXT NEGs). - PCR using 12SMiFish-U - Send to Earlham after CU2 Mock communities of 9 x DNA extracts from fish species - PCR using Aa22-PheF/Aa633-12sR primers - Sanger all 9x - Combine PCR products into 4 x mock communities - In triplicate - Re-amplify using 12SMiFish-U - Metabarcode & sequence No bioinformatics.